: In-Programme Workshops

In-Programme Workshops

14 - 16 April 2021 | BST (UTC+1)

Spatial Biology Europe: Online features four in-depth, interactive workshops from our Premium Sponsors:

Spatial Biology Applications:
Research In The Spatial And Multi Omics Era

Day One: 14 April 2021 | 14:30 – 16:00

• What is the impact of the latest technology developments in spatial genomics?
• High throughput analysis on FFPE tissue: Cancer Atlas and Whole Transcriptome
• Genomics and multi-omics: what are the best applications fit?
• Spatial deconvolution
• Tissue, single cell analysis and molecular imaging

Presentation 1: Resolving Tissue Heterogeneity: The Answers Are In The Tissue

Sarah Warren, PhD, Senior Director Translational Science, Nanostring



Presentation 2: Spatial Analysis Of The Acute Myeloid Leukaemia Microenvironment Identifies Immune Correlates Of
Therapeutic Response

Sergio Rutella, Professor of Cancer Immunotherapy, Interim Director, John van Geest Cancer Research Centre
Nottingham Trent University, UK



Presentation 3: Spatial Genomics In The Development Of Precision Immunotherapy Strategies For Lung Cancer

Jon Zugazagoitia, MD, PhD, Phycisian-Scientist in Lung Cancer, Hospital Universitario



Panel Discussion: Research in the Spatial Genomics Era

Moderator: Agnese winfield, phD, Director, Nanostring

Panellists:

  • Sergio Rutella, Professor of Cancer Immunotherapy, Interim Director, John van Geest Cancer Research Centre, UK, Nottingham Trent University, UK
  • Jon Zugazagoitia, MD, PhD, Phycisian-Scientist in Lung Cancer, Hospital Universitario
  • Sarah Warren, PhD, Senior Director Translational Science, Nanostring

Multiplex Spatial Proteomics,
Revolutionizing Tissue Imaging And Analysis

Day One: 14 April 2021 | 17:50 - 18:50

Presentation 1: Multiplex Spatial Proteomics, Revolutionizing Tissue Imaging And Analysis

Single cell analysis, starting with the earliest low parameter fluorescent experiments, helped defi ne the major cell subsets of human cellular systems as we understand them today. Now, a novel combination of elemental mass spectrometry with single cell analysis (mass cytometry, Science 2011) and imaging (multiplexed ion beam imaging – MIBI, Nature Med. 2014, Cell 2018) offers routine examination of >50 parameters without fluorescent agents or interference from spectral overlap using heavy metal isotopes as reporters. With these platforms, we have reached new levels of organizational understanding in human pathobiology. Here, we will review the evolution of these technologies and present our latest eff orts to create assays with these platforms in order to quantify multiple levels of regulation including metabolism, proteome dynamics, and chromatin content regulating the epigenome. Further to this, we will demonstrate how the ever-expanding single cell proteomics toolbox can be leveraged to understand a variety of human systems ranging from the immune to the central nervous system (CNS). Overall, these new approaches can reveal new layers of human cellular organization and structure that can be exploited to understand dysfunction in general.

Sean Bendall, Assistant Professor (Research), Department of Pathology,
Stanford University School of Medicine

Presentation 2: Relating Single Cell Function To Structure In Human Tissue Using MIBI-TOF

Understanding the role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires tools that can rapidly and consistently quantify the expression of multiple proteins while preserving spatial information. To meet this need, we have developed Multiplexed Ion Beam Imaging by Time-Of-Flight (MIBI-TOF). MIBI-TOF uses secondary ion mass spectrometry to visualize up to 50 metal labelled antibodies at subcellular resolution simultaneously with histochemical stains and native biological elements. We have used this capability to study how single cell function and tissue structure interact at the tumor immune microenvironment, the human maternal-fetal interface, and in infectious disease. These studies have led us to develop scalable, automated, and extensible tools for image thresholding, single cell segmentation, cell clustering, and cell neighborhood analyses that can be deployed on any tissue. Through NIH HuBMAP, CIMAC, and HTAN initiatives, we are currently working to deploy this computational pipeline as well as preformulated antibody staining master mixes as a streamlined, end-to-end workflow for routine high dimensional tissue analysis in basic and translational research

MICHAEL ANGELO, Assistant Professor, Department of Pathology,
Stanford University School of Medicine

Roundtable Discussion: The Future For Clinical Application Of Spatial Proteomics

Moderator: Sean Bendall, Assistant Professor (Research), Department of Pathology, Stanford University School of Medicine

Spatial Gene Expression Without boundaries - All Hands Discussion

Day Two: 15 April 2021 | 09:00 – 10:30

As genomic approaches become staples of biological research, scientific partnerships like the one at the Newcastle University between Pathology, Genomics and Bioinformatics departments will help power new discoveries. For scientists at Newcastle, collaboration was forged by each group’s common ties to Visium Spatial Gene Expression, a solution offered by 10x Genomics that enables unbiased characterization of spatially resolved whole transcriptome or targeted gene expression in thousands of spots across a section of tissue on a tissue slide.

Joint Presentation: Greater Than The Sum Of Its Parts: Newcastle University Spatial Transcriptomics Service

Jonathan Coxhead, Genomics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences
Newcastle University

Steven Lisgo, HDBR (Newcastle), Newcastle University Biosciences Institute, Faculty of Medical Sciences,
Newcastle University

Rachel Queen, Bioinformatics Support Unit, Newcastle University



Panel Discussion: Spatial Gene Expression Without Boundaries – All Hands Discussion

Moderator: Haris Tzouvali – Regional Marketing Manager, EMEA, 10x Genomics

Panellists: 

  • Jonathan Coxhead, Genomics Core Facility, Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle University,UK

  • Steven Lisgo, HDBR (Newcastle), Newcastle University Biosciences Institute, Faculty of Medical Sciences, UK

  • Rachel Queen, Bioinformatics Support Unit, Newcastle University, UK

  • Stephen Hague, Senior Science and Technology Adviser, 10x Genomics

  • Johanna Stergiadou, Science and Technology Adviser, 10x Genomics

Advances In Multiplexed Immunofluorescence To Spatially Resolved Immune Tumor Heterogeneity

Day Two: 15 April 2021 | 13:10 – 14:10

Joint Presentation:

  • Introduction to InSituPlex® DNA-barcoding and antibody staining technology for multiplex fluorescence
  • How to make use of multi-parameter data within the TME – spatial mapping, dynamic range of expression, and co-localization of markers on individual cells (Visiopharm image analysis)
  • Facilitating a streamlined image analysis workflow through CRO partners (OracleBio)

 

DAVID MASON, Technical Sales Specialist, Visiopharm

LORCAN SHERRY, Chief Scientific Officer, OracleBio

FLORIAN LEISS, Vice President Digital Health Strategies, Ultivue

KIRSTY MACLEAN, Director, Biomarker and Translational Strategies, Ultivue

Investigating Human Health And Disease At Single-Cell Resolution

Pre-Event Focus Day Workshop from our Diamond Sponsor.
03 November 2020 | 14:00 – 17:00 GMT

This complimentary workshop is bringing together a panel of worldwide experts in single genomics to discuss how human health and disease can be resolved at the single-cell level. 

Workshop chaired by: MATTHIEU PESANT, PhD, Product Manager NGS, Takara Bio, France