Presentation 1: Optimizing Process Development for Biomolecule Purification
Speaker: Dr. Hernán Alarcón
The optimal resin for your process is not always commercially available. Resin parameters are fixed by the manufacturer and may limit performance for many applications. Therefore, processes are developed within those parameters which can lead to loss of yield, throughput, or require more steps than desired to meet the production goals.
In this workshop, ABT, a manufacturer of high-quality agarose resins, will discuss how through their decades of experience, they leverage their ability to control all aspects of the resin. Case studies will be presented on the development of custom resins, that lead to higher resolution, capacity, and simplified purification processes. The result being that optimization of agarose resins is an efficient and inexpensive means to optimize your purification process development.
Presentation 2: Single Step Purification and Immobilization of His-Tag Proteins Using a Novel, Cost-Effective and Scalable Resin Technology
Speaker: Dr. Benjamin Summers
Authors: Benjamin D. Summers and Alessandra Basso
Biocatalytic processes are gaining importance in industry and enzyme immobilization is a key technique for their implementation. At the same time, the enzymes of interest need to be concentrated or isolated from the fermentation broth, and they frequently contain a histidine tag fused to their sequence so they can be purified by IMAC. In this work, we present a newly developed methacrylic iminodiacetic acid-functionalized resin, that allows an effortless capture/immobilization and biocatalytic process using a His-tagged ketoreductase. The study confirms the possibility to immobilize the enzyme in batch or flow immediately after fermentation, perform biocatalytic reactions and remove the enzyme once exhausted. Moreover, the entire process offers the opportunity for an easy scale-up, which can be difficult and expensive in other cases.
Jurgen Machielse, Director, Life Sciences AIC
Jürgen studied chemistry in the Netherlands and worked as R&D scientist and operations manager in several international environments where chromatography was the major separation technology. In 2001 he changed from being a chromatographer in leading at Varian the production of Liquid Chromatography stationary phases and consumables. After his MBA in 2007 he moved from operations to Business Development from where he specialized in implementing new departments and bringing products to the market. In this position he worked for Agilent, Osaka Soda and Zeochem. His current position is Director Life Sciences at American International Chemical (AIC) where he leads the Chromatography Department.
Hernán Alarcón, Research & Development Scientist, Agarose Bead Technologies (ABT)
Dr Hernán Alarcón is a Research & Development Scientist at Agarose Bead Technologies (ABT), where he works on the development of customized products to solve the problems that users of chromatographic techniques have. Prior to this position, he contributed to different biomedical and biotechnological projects in academic institutions, where he developed a strong background in protein engineering from genetic modification to protein production. He holds a master’s degree in Biotechnology and a PhD in Molecular Biosciences from Universidad Autónoma de Madrid.
Benjamin D. Summers, Applications Specialist, Purolite
After receiving his PhD from the University of York in Chemistry in 2014 studying industrial applications of Baeyer- Villiger monooxygenases, Benjamin Summers has focussed his early career on the use of biotransformations in commercial processes. From 2016 he has worked with Purolite Life Sciences to develop the application of immobilised enzymes and chromatographic resins to a wide range of industrial targets.
Alessandra Basso, Business Development Manager, Purolite
Alessandra Basso received her PhD in Trieste, Italy in Pharmaceutical Sciences in 2001. Her interest in immobilized enzymes started earlier in 1998 and in 2007 she founded SPRIN, a company involved in the development, manufacture and sales of resins for enzyme immobilization and ready to use immobilized enzymes for the industry. In 2012 she joined Purolite and the Life Sciences product line started with more than 600 new products developed for the industrial biocatalysis and downstream purification. Author of 70 scientific publication.